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I apologize for not starting a new subject but I couldn't find how to do it.
I have a question related to ISO 10993 though.
The norm advices to test 2 extraction procedures: one with a polar and one with a non-polar medium. As non-polar medium they give sesam oil as an example. In annex C of ISO 10993 (5), it is stated that the extract should be tested on cells at a concentration of 100%. I suppose noone could expect cells to survive an exposition to 100% sesam oil for 24h?!
How is sesam oil intended to be used then?
Should it be diluted? How much? Did I miss something?
Many thanks in advance.
I have a question related to ISO 10993 though.
The norm advices to test 2 extraction procedures: one with a polar and one with a non-polar medium. As non-polar medium they give sesam oil as an example. In annex C of ISO 10993 (5), it is stated that the extract should be tested on cells at a concentration of 100%. I suppose noone could expect cells to survive an exposition to 100% sesam oil for 24h?!
How is sesam oil intended to be used then?
Should it be diluted? How much? Did I miss something?
Many thanks in advance.