Inter/Intra assay precision study per CLSI guidelines

Karen747

Starting to get Involved
#1
Hi All,

In need of help!!

(But please direct me to the correct thread if I am in the wrong one but wasn't quite sure the right one to pick)

I don't know statistics at all really, I have precision data I need to analyse.

For example:

I have 3 analyzers and 3 different samples tested for:

For inter assay precision = 2 reps of samples, twice per day for 20 days (carried out on two lots of reagent = 80 results per lot)

For intra precision = 20 reps of each sample in on single day.
(Carried out on two lots of reagents = 40 results per lot)

I looked at CLSI guidelines which Im finding quite complicated and it says to run an ANOVA for precision data, I do have minitab but I have no idea how to use it if I am honest.

Any help would be much appreciated.

Thanks a million,

Karen
 
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Statistical Steven

Statistician
Staff member
Super Moderator
#2
Karen

The CLSI EP-05 is pretty straightforward. The statistics can be complicated, but if you are not familiar with ANOVA and variance components, I would look into hiring a consultant statistician that can assist. Simply putting the data into minitab or any software might not be enough.

Hi All,

In need of help!!

(But please direct me to the correct thread if I am in the wrong one but wasn't quite sure the right one to pick)

I don't know statistics at all really, I have precision data I need to analyse.

For example:

I have 3 analyzers and 3 different samples tested for:

For inter assay precision = 2 reps of samples, twice per day for 20 days (carried out on two lots of reagent = 80 results per lot)

For intra precision = 20 reps of each sample in on single day.
(Carried out on two lots of reagents = 40 results per lot)

I looked at CLSI guidelines which Im finding quite complicated and it says to run an ANOVA for precision data, I do have minitab but I have no idea how to use it if I am honest.

Any help would be much appreciated.

Thanks a million,

Karen
 
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