As Bev has mentioned, it depends on the variability within your process. In my past experiences, working with electro-mechanical assemblies, I often get involved and perform the function myself; of course doing this takes practice to overcome the learning curve. Another approach I have used is through interviewing operators. Inform them what and why you are doing it and ask them for a number. In most cases, they will low ball you by about 30%, but it gives you a starting point.
Depending on the number of parts per hour, you could do the old fashion way of picking an operation and using a stop watch in your pocket. This approach conceals your actions and I believe, by not alarming the operators of your actions, the result is more accurate.
The last and not least is to look at you MRP or manufacturing system and do some simple math. If your employee’s clock into jobs, the information should be ready for the taking.