# Sample Size Calculation for Image Analysis - Microscopy

#### v9991

Trusted Information Resource
typical analysis involves,

Preparation of samples(~1) --- capturing images(~ 1-10) --- analysis of images ( ~ 70-300 per image; ) - final results.( 500-1000 particle-counts)
typically we report the size of particles.

the question is, what is considered under sample size?
should it be applied to
no. of image?
no. of particles?
no. of samples?

my interpretation and rationale is that.,
no. of samples & particles is function of product performance ( finalized during development)

as part of analysis, no. of particles and images are required to be included in the analysis.

#### reynald

##### Quite Involved in Discussions
typical analysis involves,

Preparation of samples(~1) --- capturing images(~ 1-10) --- analysis of images ( ~ 70-300 per image; ) - final results.( 500-1000 particle-counts)
typically we report the size of particles.

the question is, what is considered under sample size?
should it be applied to
no. of image?
no. of particles?
no. of samples?

my interpretation and rationale is that.,
no. of samples & particles is function of product performance ( finalized during development)

as part of analysis, no. of particles and images are required to be included in the analysis.
What do you want to establish? The average size of the particles? Then the relevant sample size is the number of particles you measured.

Is it the proportion of samples that have particles? Then it is the samples that count.

#### Miner

##### Forum Moderator
Staff member
I recommend that you study the concept of an Experimental Unit. Once you define the experimental unit for your situation, that definition will answer the sample size question.

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