Hi, i would like to know what is the most appropriate method for determining bioburden for powder free surgical glove? Currently we are using the enumeration method, and would like to switch to the membrane filtration method, but during the filtration, media used (DE), is unable to pass through the filter. Its taking ages for the media to be filtered. We stomach the glove pcs, with DE (100ml), and passed through the filter, but this seems not to be working. I would appreciate any feedback or suggestion to validate the new bioburden method.