Hi,
Generally, you should not use media for removing bacteria from gloves. Media is generally thick and it will never pass through 0.3 micron membrane filter.
Instead, generally, as what we do in Pharma, use sterile water (may be WFI) with surfactant (which have no bacteriostatic action) to remove bacteria, filter it and culture the membrane in plate.
The best method is direct culturing, in your case you can cut the glove in pieces and culture it, make sure both surfaces get exposed to media.
You can refer below standard which is particularly designed for medical device, gives good idea about sterility testing
Sterilization of health care products?Microbiological methods?Part 1: Determination of the population of microorganisms on product
Annex A of this standard gives general idea about methods to be used according to types of medical device (liquid, solid, foam, powder etc.)
Also go through Annex B - "B.2.2 Removal techniques" (for microorganism)
Stomaching
Ultrasonication
Shaking (mechanical or manual)
Vortex mixing
Flushing
Blending (disintegration)
Swabbing
Eluents, diluents, and transport media
Another thing - you have to use gentle mixing process (magnetic stirrer/ shaker) - not that high speed mixer.
In my opinion - mechanical shaking/sonification/vortex/flushing are good option.
Whatever you use, make sure it is reproducible.