Which incubation condition should be selected to recover both bacteria and fungus effectively

mpfizer

Involved In Discussions
#1
HI,

Would like to know which of the two should be followed in enviornmental monitoring

(a) First incubate at 20-25°C temperature for 72 hours for fungal growth and then same plates transferred to 30-35°C for further 48 hours for bacterial growth.

(b) First incubate at 30-35°C temperature for 48 hours for bacterial growth and then same plates transferred to 20-25°C for further 72 hours for fungal growth.


These two concepts are there but which incubation condition should be selected to recover both bacteria and fungus effectively?

Thanks

Michelle
 
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indubioush

Quite Involved in Discussions
#4
Sorry, but I'm not exactly sure why. I would assume because being able to count bacteria is a higher priority. I would look at the references listed in the standard and review those materials. Or you can reach out to a consulting firm.
 
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