Which incubation condition should be selected to recover both bacteria and fungus effectively

mpfizer

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HI,

Would like to know which of the two should be followed in enviornmental monitoring

(a) First incubate at 20-25°C temperature for 72 hours for fungal growth and then same plates transferred to 30-35°C for further 48 hours for bacterial growth.

(b) First incubate at 30-35°C temperature for 48 hours for bacterial growth and then same plates transferred to 20-25°C for further 72 hours for fungal growth.


These two concepts are there but which incubation condition should be selected to recover both bacteria and fungus effectively?

Thanks

Michelle
 
Sorry, but I'm not exactly sure why. I would assume because being able to count bacteria is a higher priority. I would look at the references listed in the standard and review those materials. Or you can reach out to a consulting firm.
 
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